The method of claim 11 wherein the thromboplastin extract further comprises ethylenediaminetetraacetic acid at a concentration of at least about 10 mM. The method of claim 1 wherein the feed solution further comprises ethylenediaminetetraacetic acid at a concentration of at least about 10 mM. The present invention provides a process for preparing a thromboplastin reagent by mixing the supernatant thromboplastin extract prepared by the above process with calcium ions. A thromboplastin reagent was prepared from commercially available rabbit brain powder according to the invention as follows:. Additionally, the second surface of the membrane is typically exposed to either a carrier solution or to solvent that originates from the feed solution and passes through the membrane with the contaminant protein. In either case, the liquid exposed to the second surface of the membrane is referred to as a permeate. Hemker et al. Von Felten et al. Soria et al.
Thromboplastin or thrombokinase (TPL) is a plasma protein aiding blood coagulation through catalyzing the conversion of prothrombin to thrombin.
It is a complex enzyme that is found in brain, lung, and other tissues and especially in blood platelets and that functions in Historically, thromboplastin was a lab reagent, usually derived from placental. Tissue factor is a transmembrane protein that functions as a nonenzymatic cofactor for.
vitamin K–dependent coagulation factors, phospholipid content, and preparation. In general, thromboplastin reagents with lower ISI values are more. In general, thromboplastin reagents with lower ISI values are more Each preparation has a different sensitivity to clotting factor deficiencies and.
Therefore, recent studies focussed on possible non-hemostatic functions of this domain.
Two control assays were also evaluated -- one using a thromboplastin reagent prepared with untreated extract, but otherwise identical to the subject reagent composition, and the other using a thromboplastin reagent prepared with an untreated extract dialyzed against a 12, Dalton 12 kD MWCO membrane, but otherwise identical to the subject reagent composition.
McDonagh et al.
Chymotrypsin is a preferred proteolytic enzymes. Studies on the adsorption and activation of the Hageman factor factor XII by collagen and elastin.
Thromboplastin extract, reagent, and preparation thereof Helena Laboratories, Corp.
The thromboplastin reagent may contain a metal ion chelator if calcium ions are present in sufficient concentration to overcome the metal ion chelator level in addition to the amount of sodium citrate or EDTA present in the anticoagulated plasma.
Tripodi et al. Such semipermeable membranes are preferably solid membranes and are typically polymeric in nature, but are not limited to such for purposes of the present invention.
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|Such a quantitative determination involves determining the clotting times of standard plasmas having various known levels of the plasma clotting factor and preparing a standard correlation between the clotting times of the standard plasmas and the level of the plasma clotting factor in the standard plasmas.
See Perry's Chemical Engineer's Handbook, supra. Fibrin is formed from a soluble precursor, fibrinogen, by the protease thrombin. The extrinsic and intrinsic pathways converge in a final common pathway for the thrombin-catalyzed conversion of fibrinogen to the fibrin clot. Break up of this EPI complex by chelators appears to allow isolation of thromboplastin free from the inhibitory EPI, assuming that the EPI partitions into the pelleted rabbit brain residue upon separation, for example, centrifugation.
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USA Thromboplastin extract, reagent, and preparation thereof Google Patents
STABILIZED THROMBOPLASTIN PREPARATION. Any metal ion chelator that functions similarly to ethylenediaminetetraacetic acid (EDTA) in. thromboplastin reagents vary widely in composition and manner of preparation. on the amino terminus has no effect on rTF function . Reconstitution of rTF.
Because thromboplastin reagents vary widely in composition and manner. peptide on the amino terminus has no effect on rTF function .
Calcium salts of sugar acids such as calcium tartrate, calcium gluconate, calcium citrate, or calcium lactate may be substituted for calcium chloride. The thromboplastin reagent can be maintained in any form in which it will retain its procoagulant activity.
Preferably, a small amount of pH neutralized, concentrated metal ion chelator is delivered to the extraction solution prior to adding the powdered source of thromboplastin.
The method of claim 30 further comprising the step of exposing a carrier solution to a second surface of the membrane. Doolittle et al. A first recirculation pump recirculates a first stream through a first separation unitwith the permeate being discharged through a combined discharge header, the bulk of the retentate being recirculated through the loop and a relatively small portion of the retentate being bled off to supply a second recirculation loop From time to time, for as yet unknown reasons, rabbit brain powder from commercial suppliers may vary from its normal quality, and produce extracts unsuitable for reagent preparation by conventional methods because they yield long PTs for normal human plasma.
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|Jensen et al.
Video: Thromboplastin reagent composition of functions Composition of Functions
The three general steps in preparing a thromboplastin reagent include preparing a powdered thromboplastin source, converting the powdered thromboplastin source into a thromboplastin extract, and preparing a useful thromboplastin reagent from the thromboplastin extract.
PTE en. Other reagent components such as buffers, stabilizers, preservatives, sensitizing agents and bulking agents can also be included in the thromboplastin reagent composition. The present invention also provides a method for stabilizing a thromboplastin extract by combining the thromboplastin extract with a phospholipase inhibitor.